Antimicrobial liquid compositions and methods for using them

ABSTRACT

A liquid composition for applying a non-leachable antimicrobial coating on a surface. The liquid composition consists of a solution, dispersion or suspension of a biguanide polymer reacted with a cross-linking agent to form an adduct, and an antimicrobial metal material. The resulting antimicrobial coating does not release biocidal levels of leachables into surrounding solution.

This application 371, filed Dec. 19, 1994, of International applicationno. PCT/US94/14636, which is c.i.p. of U.S. Ser. No. 08/220,821, filedMar. 31, 1994 (now abandoned) which is a c.i.p. of U.S. Ser. No.08/170,510, filed Dec. 20, 1993, (now issued as U.S. Pat. No. 5,490,938.

FIELD OF THE INVENTION

The present invention relates to liquid dispensers, specifically, theprovision of liquid dispensers capable of maintaining the sterility ofsterile solutions during storage, during dispensing, and subsequent todispensing of the solution, as well as methods of manufacture and use ofsuch dispensers.

BACKGROUND OF THE INVENTION

Many indications require administration of sterile solutions. Ingeneral, such solutions, and the dispensers in which they are stored,are sterilized prior to closure of the dispenser. Contamination canoccur, however, after the dispenser is opened and used. Variousapproaches have been employed in attempts to minimize this contaminationproblem.

Single dose dispensers are available. Such dispensers, however, are madeonly for one time use, and then are discarded, adding considerably topackaging costs and waste. Moreover, more sterile solution than isrequired for a single dose usually is packaged which adds to the expenseof the treatment. Another problem is that persons may attempt to use thesingle dose dispenser multiple times, which can result in contaminationof the liquid being dispensed.

Alternatively, preservatives have been added to multi-dose dispensers toprevent microbial contamination after the dispenser is initially used.Such preservatives, however, often are toxic to mammalian cells, as wellas microbial cells. For example, many preservatives used in eye dropformulations are toxic to the goblet cells in the eye. Such toxicity isdetrimental to persons requiring prolonged application of the solutions.Moreover, persons often develop chemical sensitivity to thepreservative, resulting in significant allergic reactions to thepreparations. Such allergies can appear in some persons after prolongedexposure, as well as in others after only a single exposure.

Membrane filters have also been used in liquid dispensers in attempts toprevent microbial contamination of the stored sterile liquid. If ahydrophilic filter is used, however, the filter can allow the phenomenonknown as "grow-through," in which microbial progeny on the downstream(non-sterile) side of the filter can pass through the filter poresbecause of their smaller size during cell division, and therebycontaminate the sterile solution contents in the dispenser.

Hydrophobic filters have been employed in liquid dispensers. Hydrophobicsurfaces are non-wetting, and therefore are significantly more difficultfor microbes to grow on. Such filters, however, because of theirhydrophobicity prevent the flow of sterile aqueous solutions through thefilter.

SUMMARY OF THE INVENTION

It is an object of the invention to provide a multi-dose liquiddispenser which prevents external microbial contamination of the liquidduring repeated use.

According to the invention, an article of manufacture is provided--aliquid dispenser for dispensing a sterile liquid. The liquid dispensercomprises a container for storing the sterile liquid and a nozzleassembly which is attached to the container. The nozzle-assembly has apassageway which enables the sterile liquid to flow from the containerthrough the passageway in order to dispense the liquid. The liquiddispenser further comprises an integral, non-leaching antimicrobialelement for inhibiting microbial contamination of the solution. In oneembodiment, the antimicrobial element comprises a filter or filtersattached to the nozzle assembly and positioned across the passageway sothat liquid and air flow are directed through the filter.

The filter comprises a substrate having an antimicrobial agent attachedto the surface and in the pores thereof. The substrate may be an organicor inorganic material. The antimicrobial agent can be a metallic ornon-metallic material having anti-bacterial, anti-viral and/oranti-fungal properties, or a combination thereof. In one embodiment, thefilter is at least partially coated on the downstream surface with ametallic material, e.g., a metal, metal oxide, metal salt, metalcomplex, metal alloy, or mixtures thereof, which are bacteriostatic orbacteriocidal. The filter has pores of a size which precludes passage ofmicroorganisms through the filter while permitting passage of thesterile liquid from the container through the filter. The pores arepreferably approximately 0.1 microns to approximately 1.2 microns, andmore preferably approximately 0.22 microns to approximately 0.65 micronsin diameter.

In another embodiment, the upstream surface of the filter may be atleast partially coated with the antimicrobial material.

In another embodiment, the surfaces and plurality of pores of the filterare at least partially coated with an additional differentbacteriostatic or bacteriocidal material. A variation is the liquiddispenser having a second filter that is serially aligned with the firstmetal coated filter, and which is at least partially coated on at leastone surface and within a plurality of its pores with a differentbacteriostatic or bacteriocidal material.

In certain embodiments, the filter and a plurality of the pores can haveat least a partial coating with a non-metallic antimicrobial compoundthat has an anti-viral, anti-fungal or anti-bacterial property. Thenon-metallic antimicrobial compound may be used in lieu of or inaddition to the metallic materials.

In another embodiment, the filter includes a hydrophobic portion forallowing air to enter the container to replace the sterile liquid thatis dispensed from the filter dispenser. In another variation, thedispenser may contain a second port separate from the dispensing nozzlefor allowing replacement air into the container after the liquid isdispensed. In order to ensure the sterility of the air entering thissecond port, the port opening would be spanned by a hydrophobic membranehaving a pore size that precludes bacterial migration into thedispenser, or having an antimicrobial agent attached thereto or coatedthereon.

In yet another embodiment of the invention, the passageway walls in thenozzle assembly, at least on the downstream side of the filter, arecoated with an antimicrobial material that is bacteriostatic orbacteriocidal. The antimicrobial material may be any of the metallic ornon-metallic antimicrobial materials described herein.

In other variants, the liquid dispenser can have a prefilter which isspaced upstream from the filter for providing a barrier to the passageof particulate matter through the prefilter and for permitting thepassage of sterile liquid from the container through the prefilter. Asupport means can also be spaced upstream from the filter forreinforcement of the filter.

In another embodiment of the present invention, the dispenser maycontain an antimicrobial element in lieu of or in addition to thefilter, the surface of which is at least partially coated with anantimicrobial agent. The element is disposed within the body of thecontainer such that it remains in contact with the sterile solution atall times, e.g., during storage and dispensing. This is accomplished byproviding a substrate having permanently attached thereto or coatedthereon an antimicrobial agent. The substrate may be a bead or pluralityof beads, a membrane, cartridge, filter, wool, cotton, baffle or fibrousbundle, for example. The element may be free-floating in the solutionwithin the container or may be attached to or immobilized within thecontainer. In this embodiment, the antimicrobial element remains incontact with the solution thereby insuring its sterility even afterrepeated doses have been dispensed by a user.

In another aspect of the above embodiment, the inside wall of thecontainer which is in contact with the solution is coated with or hasattached thereto an antimicrobial material.

Another aspect of the invention provides a membrane, element or surfacewhich has an antimicrobial material coated thereon or attached thereto.In one embodiment, a microporous membrane having pores or otherperforations which provide liquid conduits interconnecting the upstreamand downstream surfaces of the membrane for liquid passage from onesurface to the other is treated such that at least one surface and atleast some of the pores are coated or otherwise derivatized with theantimicrobial material. The pores are of a size so as to precludepassage of microorganisms through the membrane and so as to permitpassage of liquid and air through the membrane. Variations include allsurfaces, including the pores, being at least partially coated with theantimicrobial material. The membrane surfaces and a plurality of thepores can be at least partially coated with an additional antimicrobialmaterial that has an anti-viral, anti-fungal or anti-bacterialproperties.

The invention also includes a method in which a liquid can be dispensedby applying pressure to the container of the liquid dispenser of thisinvention so as to discharge the liquid from the container. Thecontainer preferably is formed at least in part of a resilientlydeformable material, such as an elastic polymer, which permits manualsqueezing to discharge a dose of medicament, and subsequent elasticrecovery of the material to its original configuration by drawing gasfrom a surrounding atmosphere into the container while the gas issterilized by the filter in passing therethrough.

In a preferred embodiment of the invention, the liquid dispenser is usedfor eyecare in an individual, in which a sterile eyecare liquid, e.g.,liquid artificial tears, a solution for contact lens care or amedicament, is dispensed from the liquid dispenser into the eye or ontoan object that is to be placed into the eye. Preferably, the eyecareliquid is preservative-free.

The invention also features methods for attaching antimicrobial agentsto the surfaces of a substrate. This may be accomplished by a number ofmethods. For example, metallic compounds may be applied to a surface bymetal vapor deposition, electroless plating, chemical derivatization orcoating. Non-metallic antimicrobial materials may be applied to suchmetallic surfaces by chemical derivatization or coating, for example.

In one embodiment, a metallic silver coating is accomplished bycontacting the substrate with a carbonyl compound, e.g., an aldehydesuch as glutaraldehyde, a sugar such as glucose, or an aldehydefunctionality generating compound, drying the substrate, contacting thedried substrate with a metal salt, e.g., silver nitrate, or metalcarboxylate salt solution, e.g., silver tartrate, and anamine-containing compound solution, e.g., ammonium hydroxide, so as todeposit the metal on the surface and within a plurality of the pores ofthe substrate. In an alternative embodiment, the drying step is omitted.One or more techniques may be combined to accomplish the desired result.For example, metal vapor deposition deposits metal on a surface of amembrane, but not in the pores. Therefore, antimicrobial material can bedeposited in the pores of the membrane by an appropriate technique,followed by metal vapor deposition to coat the surface, thereby forminga membrane in which both the pores and the surface are coated withantimicrobial material.

In another embodiment, the substrate is contacted with an activator,e.g., a tin dichloride solution, is dried, and then contacted with ametal salt or metal carboxylate salt solution, either with or without anamine-containing compound solution, so as to deposit the metal on thesurfaces of the substrate.

In another embodiment of the present invention, non-metallicantimicrobial agents are covalently attached to or coated onto a metalcoated substrate such as a filter or an element disposed in thereservoir of the container. Non-metallic antimicrobial agents mayinclude any anti-bacterial, anti-viral and/or anti-fungal materialswhich are capable of being immobilized on a surface and which arecompatible with the sterile liquid. Most preferred are the class ofagents which cause dissolution of the lipid bilayer membrane of amicroorganism. For this purpose, surface active agents, compounds suchas cationic or polycationic compounds, anionic or polyanionic compounds,non-ionic compounds and zwitterionic compounds may be used. Preferredagents include biguanide compounds or benzalkonium compounds. Theseagents may be attached to the substrate by covalent bonding, ionicinteraction, coulombic interaction, hydrogen bonding, crosslinking(e.g., as crosslinked (cured) networks) or as interpenetrating networks,for example.

In another embodiment of the present invention, non-metallicantimicrobial agents are covalently attached to or coated onto asubstrate such as a membrane, filter, an element disposed in thereservoir of the container or the walls of the reservoir in contact withthe solution. These non-metallic agents are attached or coated directlyonto the surface of the substrate in lieu of the metal coating.Non-metallic antimicrobial agents useful for his purpose include anyanti-bacterial, anti-viral and/or anti-fungal materials which arecapable of being immobilized on a surface and which are compatible withthe liquid. Most preferred are the class of agents which causedissolution of the lipid bilayer membrane of a microorganism. For thispurpose, surface active agents, compounds such as cationic orpolycationic compounds, anionic or polyanionic compounds, non-ioniccompounds and zwitterionic compounds may be used. Preferred agentsinclude biguanide compounds or benzalkonium compounds. These agents maybe attached to the substrate by covalent bonding, ionic interaction,coulombic interaction, hydrogen bonding or interpenetrating networks,for example.

Articles made in accordance with these methods are also included in thisinvention.

A multidose dispenser containing a hydrophilic filter having immobilizedthereon an antimicrobial agent that prevents bacterial grow-throughwhile maintaining high flow rates of aqueous solutions was unknown inthe art prior to Applicants' invention.

The present invention is unique in the following respects:

i) A multi-dose dispenser that incorporates a hydrophilic membrane whichis surface modified (including pores) with a bound antimicrobial agent.

ii) The ability of the filter to prevent microbial grow through in longterm contact applications, while maintaining high flow rates of aqueoussolutions.

iii) The unique nature of the antimicrobial agent that utilizes asynergistic effect of it's components. This results in surface highbiocidal activity, while maintaining almost no significant leachablesinto solutions it is in contact with

iv) The mechanism of action being essentially a surface mediated one,whereby organisms succumb only upon contact with the filter due to thenon leaching property associated with it.

v) The ability of such surfaces to remain viable over multiple organismchallenges with no decrease in their bioactivity.

vi) The utilization of such biocidal coatings on the dispensing tip ofthe device, thereby eliminating the possibility of microbialcolonization in the dead volume of the tip downstream to the filter.

vii) User friendliness and cost effectiveness of the device for alltypes of applications.

viii) Adaptability to existing manufacturing technology, therebyenabling large scale manufacture without added cost.

ix) Applicability to a variety of ophthalmic formulations over a widerange of solution viscosity including artificial tears, saline,anti-glaucoma and ocular hypertensension drugs, and contact lenscleaning solutions.

x) Readily adaptable for varied flow requirements (single drop orstream).

xi) Readily adaptable for the delivery of other types of medicaments orsolutions where preservatives have been used such as ear and nasal drugformulations.

The above and other objects, features and advantages of the presentinvention will be better understood from the following specificationwhen read in conjunction with the accompanying drawings.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 is a semi-diagrammatic cross-sectional view of a liquid dispenserin accordance with the present invention.

FIG. 2 is a semi-diagrammatic cross-sectional view of an upper portionof the liquid dispenser of FIG. 1.

FIG. 3 is a semi-diagrammatic top view taken through line 25--25 of FIG.2 showing the concentric and radial channels of the invention.

FIG. 4 is a semi-diagrammatic cross-sectional view of a liquid dispensercontaining anti-microbial coated beads in accordance with the presentinvention.

FIG. 5 is a semi-diagrammatic cross-sectional view of a liquid dispenserof FIG. 4 containing antimicrobial coated fibers.

DETAILED DESCRIPTION

One embodiment of the present invention, as shown in FIGS. 1-3, providesa liquid dispenser 1 for dispensing a sterile liquid 2. The liquiddispenser 1 has a container 4 for storing sterile liquid 2 and a nozzleassembly 3 which is mounted on top of container 4. Nozzle assembly 3 hasa passageway 5 which enables sterile liquid 2 to flow from container 4through passageway 5 when sterile liquid 2 is dispensed.

Container 4 is designed to permit manual squeezing so as to forcesterile liquid 2 from container 4 through filter 6 out of orifice 7 ofnozzle assembly 3. In normal operation, liquid dispenser 1 is invertedand container 4 is squeezed.

Container 4, as shown in FIG. 1, has a circular cross-section extendingalong a vertical axis 8, with a flat bottom 9 and an upper end 10.Sidewall thickness is preferably in the range of 0.01 to 0.25 inch.However, various sizes and configurations can be used. The shape of thecontainer can be round, elliptical, polygonal, irregular, or the like,and in some cases may be in tube form. The particular sidewall thicknesscan vary greatly, as can the volume of the chamber within container 4that holds the medicament or other liquid to be dispensed. Thus, varioussizes ranging from cubic millimeters to cubic centimeters or more can beused for the container chamber.

In a preferred embodiment, the nozzle assembly 3 is an ovoid form. Thenozzle assembly has a cross-section formed of a plastic material whichis self-supporting and defines a generally ovoid configuration having aninverted lip portion 11 mating with and sealed to the top of container 4at upper end 10. Nozzle assembly 3 includes within it a ring-shapedlower spacer 12 of a solid material having a central passage connectingthe chamber of container 4 with the filter or filters and an upperspacer 13 which acts to hold the filter or filters in place. The lowerspacer 12 comprises a supporting screen 30. A disc 14 carries channelmeans. As shown in FIG. 3, disc 14 carries a plurality of concentricchannels 22 which are interconnected by radial channels 23 to a centralpassageway 5 so that liquid coming from container 4 will pass throughthe filter or filters and be distributed on the surface of disc 14 so asto cause dispensed sterile liquid 2 to coalesce into a single drop or astream of liquid when expelled from container 4. Depending on factorsincluding, e.g., the applied pressure, the viscosity of the expelledliquid and the surface area of disc 14, either a single drop or a streamof liquid will be dispensed. Disc 14 is held in place by beingadhesively secured, e.g., by ultrasonic welding or by a mechanicalforce, to the upper spacer 13. Support 16, prefilter 15, filter 6 andsecond filter 17 can be suspended by spacers 12 and 13. In someembodiments, only filter 6 need be used and one or more of the support,prefilter or second filter, can be eliminated. Various combinations ofthese elements can be used in different embodiments as desired.

While the prefilter and filter, as well as the second filter, are shownas planar members, various configurations can be used. These members canbe in the form of cones, polygonal or other shaped devices as may bedesirable for specific applications. Planar sheet-type materials asshown are most preferred.

While passageway 5 is preferably axially extending with a circularcross-section, it can have any configuration as desired for specificapplications.

Container 4 can be formed from a flexible material; e.g., an elasticallydeformable polymer which may be a thermosetting or thermoplasticpolymeric material, including, for example, polypropylene, polyethylene,polyvinylchloride, polyethylene terephthalate, polytetrafluoroethylene,polysulfone and polyethersulfone polymers or copolymers. In some casesthe container can be a deformable metallic or plastic medicamentcontainer, such as a toothpaste tube, where the container may remaindeformed after each dose is dispensed.

Nozzle assembly 3 can be formed from the same or a more rigid type ofmaterial than container 4. In one embodiment, nozzle assembly 3 ispermanently attached to container 4 with a liquid-tight connection so asto aid in maintaining the sterility of sterile liquid 2 in container 4.Such a connection can be formed by standard techniques, e.g., ultrasonicwelding, heat press sealing, adhesive sealing or mechanical sealing.

Filter 6 is sealingly attached to nozzle assembly 3 so that filter 6extends across the entire expanse of passageway 5 to direct liquid andair flow out of and into containers through filter 6. Filter 6 can beattached to nozzle assembly 3 by any method which results in such aseal, including, e.g., ultrasonic sealing, heat press sealing andadhesive sealing.

By filter is meant any material which can function as a microbialfilter. Microporous membranes are preferred filter materials. As usedherein, the term "microporous" means having pores of an average diameterof 5 m or less. Membranes used in the filter of this invention may beformed from organic or inorganic materials. Organic materials includepolymeric materials which can be used for the preparation of membranesor filter papers. Examples of organic polymeric materials includepolysulfone, polyethersulfone, polyamide (e.g., nylon), polycarbonate,polyacrylate, polyvinylidene fluoride, polyethylene, polypropylene,cellulosics (e.g., cellulose), and Teflon®. The hydrophobic materials,e.g., polypropylene or Teflon®, may require prior surface activationwith techniques such as plasma, chemical oxidation or metallicsensitization, e.g., a primer. Inorganic filters include glass fiberfilter paper, ceramic membranes (e.g., alumina or silica), and metalfilters. Sintered glass and sintered ceramic blocks also can be used.The filters can be either hydrophilic or hydrophobic. If a hydrophobicfilter is used, the metal coating, described below, converts it to afilter with hydrophilic properties.

Filter 6 has pores which form interconnecting liquid conduits extendingfrom an upstream surface of the filter to a downstream surface. The poresize for filter 6 is chosen so that the pores permit passage of sterileliquid 2 from container 4 through filter 6, but preclude passage ofmicroorganisms through filter 6, thereby maintaining the sterility ofsterile liquid 2 in container 4. By microorganism is meant bacteria,blue-green algae, fungi, yeast, mycoplasmids, protozoa and algae. Thepore size can range from approximately 0.1 microns to approximately 1.2microns. Preferably, the pore size is approximately 0.22 microns toapproximately 0.65 microns. Most preferably, the pore size is about 0.65microns. Whereas 0.22 microns is the pore size used in most bacterialfiltration systems, this invention can produce a sterile filtrate withlarger pore sizes, e.g., 0.45 and 0.65 microns, thus permitting a devicewhich gives a faster flow rate for the dispensed liquid.

A major problem in multi-dose liquid dispensers is that residual liquidmay accumulate downstream of the filter subsequent to dispensing liquidfrom the container. By downstream of the filter is meant the side of thefilter that liquid from the container which has passed through thefilter would be on, e.g., the surface of the filter exposed to theoutside atmosphere. By upstream of the filter is meant the side of thefilter facing the liquid in the container which has not yet passedthrough the filter. Microorganisms can multiply in this accumulateddownstream liquid and contaminate liquid which is subsequentlydispensed. Moreover, certain microorganisms in this accumulated liquidcan, because of their smaller size during cell division, pass throughthe pores of the filter, a phenomenon known as "grow-through," andcontaminate the sterile liquid in the container.

This invention addresses this problem by providing that filter 6 be atleast partially coated with or has attached thereto on the downstreamsurface and within a plurality of the pores with an antimicrobialmaterial that is bacteriostatic or bacteriocidal. By bacteriocidal ismeant the killing of microorganisms. By bacteriostatic is meantinhibiting the growth of microorganisms, which can be reversible undercertain conditions. The antimicrobial agent may be a metal or metalcompound, a non-metallic compound, or a combination of both.

In a preferred embodiment, the antimicrobial agent is a metal, metaloxide, metal salt, metal complex, metal alloy or mixture thereof whichis bacteriocidal or bacteriostatic. By a metallic material that isbacteriostatic or bacteriocidal is meant a metallic material that isbacteriostatic to a microorganism, or that is bacteriocidal to amicroorganism, or that is bacteriocidal to certain microorganisms andbacteriostatic to other microorganisms. In certain embodiments, filter 6is also at least partially coated on the upstream surface with ametallic material, e.g., a metal, metal oxide, metal salt, metal complexor metal alloy or mixtures thereof. Any metal which is bacteriostatic orbacteriocidal can be used. Examples of such metals include, e.g.,silver, zinc, cadmium, mercury, antimony, gold, aluminum, copper,platinum and palladium. The appropriate metal coating is chosen basedupon the use to which the sterile liquid passing over the metal coatedfilter is to be put. Preferably, metals which are not toxic are attachedto filters which are to be used for filtering solutions that are to beapplied to humans and other organisms. The currently preferred metal issilver.

In another embodiment of the present invention, filter 6 is first coatedwith an antimicrobial metal, metal salt or metal complex material, and anon-metallic antimicrobial agent is covalently attached to or coatedonto the metal coated substrate. Non-metallic antimicrobial agentsuseful for this purpose include any anti-bacterial, anti-viral and/oranti-fungal materials which are capable of being immobilized on asurface and which are compatible with the sterile liquid. Most preferredare the class of agents which cause dissolution of the lipid bilayermembrane of a microorganism. For this purpose, surface active agents,compounds such as cationic or polycationic compounds, anionic orpolyanionic compounds, non-ionic compounds and zwitterionic compoundsmay be used. Preferred agents include biguanide compounds orbenzalkonium compounds. These agents may be attached to the substrate bycovalent bonding, ionic interaction, coulombic interaction, hydrogenbonding, crosslinking (e.g., as crosslinked (cured) networks) or asinterpenetrating networks, for example.

In another embodiment of the present invention, filter 6 is treated withnon-metallic antimicrobial agents which are covalently attached to orcoated onto the surfaces and/or pores of the filter. These non-metallicagents are attached or coated directly onto the surface and/or pores ofthe substrate in lieu of the metal coating. Non-metallic antimicrobialagents useful for his purpose include any anti-bacterial, anti-viraland/or anti-fungal materials which are capable of being immobilized on asurface and which are compatible with the liquid. Most preferred are theclass of agents which cause dissolution of the lipid bilayer membrane ofa microorganism. For this purpose, surface active agents, compounds suchas cationic or polycationic compounds, anionic or polyanionic compounds,non-ionic compounds and zwitterionic compounds may be used. Preferredagents include biguanide compounds or benzalkonium compounds. Theseagents may be attached to the substrate by covalent bonding, ionicinteraction, coulombic interaction, hydrogen bonding, crossinking (e.g,as crosslinked (cured) networks) or as interpenetrating networks, forexample.

In another embodiment of the present invention, filter 6 is treated withnon-metallic antimicrobial agents which are covalently attached to orcoated onto the surfaces and/or pores of the filter. These non-metallicagents are attached or coated directly onto the surface and/or pores ofthe substrate. Non-metallic antimicrobial agents useful for this purposeinclude any anti-bacterial, anti-viral and/or anti-fungal materialswhich are capable of being immobilized on a surface and which arecompatible with the liquid. Most preferred are the class of agents whichcause dissolution of the lipid bilayer membrane of a microorganism. Forthis purpose, surface active agents, compounds such as cationic orpolycationic compounds, anionic or polyanionic compounds, non-ioniccompounds and zwitterionic compounds may be used. Preferred agentsinclude biguanide compounds or benzalkonium compounds. These agents maybe attached to the substrate by covalent bonding, ionic interaction,coulombic interaction, hydrogen bonding, crosslinking (e.g. ascrosslinked (cured) networks) or as interpenetrating networks, forexample. An antimicrobial metal, metal salt or metal complex material isintroduced into the non-metallic antimicrobial coating either prior toor after coating the surface in the form of either as particles or as ahomogeneous solution.

In one embodiment, filter 6 is a membrane having both hydrophilic andhydrophobic regions. For example, a hydrophobic filter which has onlybeen coated with a metal or metal oxide or metal salt on a portion ofthe filter, will be hydrophilic for the coated portion and hydrophobicfor the uncoated portion. In another example, a hydrophilic orhydrophobic filter is coated with a metal, metal oxide or metal salt, soas to make the filter hydrophilic, and then a portion of this metallicsurface is rendered hydrophobic by incorporation of a hydrophobiccoating via formation of a spontaneously self-assembled monolayer thatis covalently attached to the metallic surface, e.g., formation of analkyl thiolate monolayer on a silver coated surface by treatment with asolution of an alkyl thiol, such as dodecane thiol. In another example,a portion of a hydrophilic filter may be rendered hydrophobic bytreatment with a polymeric siloxane, a perfluoro polymer, or silylmonomer(s) or perfluoro monomer(s) that may be polymerized thermally,photolytically or chemically. Such a dual purpose filter is preferredwhen multiple doses of liquid are dispensed in quick succession to eachother, in order to more quickly replace the liquid which has beendispensed from the container with air from outside the container, so asto equalize the pressure. The dispensed liquid can pass through thehydrophilic portion of the filter, and the replacement air can passthrough the hydrophobic portion without being hampered by the presenceof liquid on the hydrophilic portion.

In the embodiment of FIG. 2, an air port or vent (not shown) can beprovided through upper spacer 13 positioned directly above thehydrophobic portion of the filter so as to allow air passage tocontainer 4 as the liquid is dispensed from container 4. The air port orvent provides for compensation of the air pressure as liquid isdispensed from the container so as to avoid formation of a vacuum. Thedevice will work with or without the air port or vent, however, if aconstant and sustained flow is desired, better flow rates may beobtained with the use of an air port or vent. In those cases where ahydrophobic/hydrophilic membrane is used, the air port or vent describedmay be particularly desirable to equalize pressure as liquids leave thecontainer. In such a case, it is preferred that the air port or vent bepositioned above the hydrophobic portion of the filter.

The invention also provides for a filter in which the downstream surfaceand a plurality of the pores are at least partially coated with anadditional second antimicrobial material. In one embodiment, theupstream surface is also at least partially coated with a second metal,metal oxide, metal salt, metal complex or metal alloy, or mixturethereof. Examples of metals that can be used are discussed above inrelation to the single metal coating. The use of two different metalscan enhance the antimicrobial properties of the filter. Different typesof microorganisms can exhibit different degrees of sensitivity todifferent metals. In addition, the use of two different metals cansignificantly reduce the problem of selection for microorganisms havingresistance to the metal in the metal coating that can occur when onlyone metal is used.

Another variation of the invention is a liquid dispenser which has asecond filter 17 with pores of a size that permits passage of sterileliquid 2 from container 4, that is serially aligned with filter 6.Second filter 17 is at least partially coated on at least one surfaceand within a plurality of its pores with a different antimicrobialmaterial, e.g., a metal, metal oxide, metal salt, metal complex or metalalloy or mixtures thereof, that is bacteriostatic or bacteriocidal, thanis used for the coating on filter 6. The presence of differentantimicrobial materials on different filters in the liquid dispenser isadvantageous for the same reasons as discussed above regarding theembodiment where two different antimicrobial materials are applied to asingle filter. In other embodiments, more than two differentantimicrobial filters are present.

In another embodiment of this invention, the surfaces of the filter (orother membrane) and a plurality of pores of the filter or membrane iscoated with or has attached thereto, a non-metallic compound that hasantimicrobial properties, e.g., antiviral, antibacterial and/orantifungal properties. By anti-viral is meant capable of killing, orsuppressing the replication of, viruses. By anti-bacterial is meantbacteriostatic or bacteriocidal. By antifungal is meant capable ofkilling or suppressing replication of fungi. This non-metallicantimicrobial material may be used in lieu of or in addition to themetallic coating. Use of these materials in conjunction with themetallic agent as an additional coating can allow for more effectiveanti-bacterial liquid dispensers, in that different anti-bacterialcompounds can exhibit different degrees of effectiveness for differenttypes of microorganisms. Multiple anti-bacterial compounds alsosignificantly reduce the problem of selection for microorganisms havingresistance to the metal in the metal coating, as discussed above.Moreover, a combination of antimicrobial materials can allow for jointanti-bacterial/anti-viral/anti-fungal liquid dispensers. Preferably,this compound is bound to at least a portion of the first antimicrobialcoating on the filter. Any compound which has anti-bacterial,anti-fungal or anti-viral activity can be used. Examples of suchcompounds include cationic or polycationic compounds, anionic orpolyanionic compounds, non-ionic compounds and zwitterionic compounds.Preferred compounds include benzalkoniumchloride derivatives (see, forexample, Example 9), a-4- 1-tris(2-hydroxyethyl) ammonium-2-butenyl!poly1-dimethylammonium-2-butenyl!-ω-tris(2-hydroxyethyl)ammonium chloride,and biguanides of the general formula: ##STR1## or their water solublesalts, where X is any aliphatic, cycloaliphatic, aromatic, substitutedaliphatic, substituted aromatic, heteroaliphatic, heterocyclic, orheteroaromatic compound, or a mixture of any of these, and Y₁ and Y₂ areany aliphatic, cycloaliphatic, aromatic, substituted aliphatic,substituted aromatic, heteroaliphatic, heterocyclic, or heteroaromaticcompound, or a mixture of any of these, and where n is an integer equalto or greater than 1. Preferred compounds include, e.g., chlorhexidineor polyhexamethylene biguanide (both available from Zeneca ofWilmington, Del.). These compounds may be modified to include a thiolgroup in their structure so as to allow for the bonding of the compoundto the metallic surface of the filter. Alternatively, these compoundsmay be derivatized with other functional groups to permit directimmobilization on a non-metallic surface. For example, theabove-mentioned antimicrobials may be suitably functionalized toincorporate groups such as hydroxy, amine, halogen, epoxy, alkyl oralkoxy silyl functionalities to enable direct immobilization to thesurface in lieu of a metal.

Antimicrobial elements having the various antimicrobial compounds coatedor attached thereon described above also are included in this invention.

The invention also includes an embodiment in which the liquid dispenserhas a prefilter 15 which is spaced upstream from filter 6 and provides abarrier to the passage of particulate matter through prefilter 15, whilepermitting passage of sterile liquid 2 from container 4 throughprefilter 15. In this manner, particulate matter that may be present insterile liquid 2 in container 4 does not need to be filtered by filter6, and thus prevents clogging of filter 6, thereby aiding in preservingthe capacity of, and flow rate through, filter 6. Preferably, the poresize of prefilter 15 is approximately 1 micron to approximately 50microns. The prefilter material includes, e.g., glass fibers, syntheticpolymer fibers, e.g., hydrophilic polypropylene fibers, nylon andcellulosic fibers. Preferably, prefilter 15 is attached to filter 6 inembodiments where there is only one filter, or attached to the mostupstream filter where there is more than one filter, and is alsoattached to nozzle assembly 3. Preferably, the attachments are bywelding.

In another embodiment, the liquid dispenser has a support 16 which isspaced upstream from the filter to act as a reinforcement for thefilter. Preferably, support 16 is perforated. Support 16 can be madefrom any material that the container or nozzle assembly is made from.

In other embodiments of the invention, the internal walls 18 of nozzleassembly 3 are at least partially coated, with an antimicrobial agent Asdescribed herein above, the agent may be a metallic material, e.g., ametal, metal oxide, metal salt, metal complex, metal alloy or mixturesthereof, or may be a non-metallic organic material that isbacteriostatic or bacteriocidal or a combination of the two. After theliquid dispenser of the invention has initially been used to dispenseliquid from container 4, some residual liquid may remain on internalwalls 18 of nozzle assembly 3 downstream from filter 6. Microorganismscan grow in this residual liquid and contaminate any future drops ofliquid which are dispensed. By coating these walls with ananti-bacterial material, this contamination is reduced. Examples ofmetals and non-metallic materials which can be used to coat the nozzleassembly walls were discussed above in relation to coating filter 6.Example 12 describes a method for depositing silver onto the walls ofthe nozzle assembly.

In another preferred embodiment, shown in FIGS. 4-5, the container 4 hasdisposed therein an antimicrobial element in contact with sterile liquid2. In this embodiment, the antimicrobial element may be present in lieuof or in addition to the filter. The element comprises an organic orinorganic substrate having an antimicrobial agent attached thereto ascoated thereon. The substrate may have any shape or configuration andmay be free-floating within the container or may be attached to or be anintegral part of the container. In the embodiment shown in FIG. 4, thesubstrate comprises beads 32. In the embodiment shown in FIG. 5, thesubstrate comprises a cotton ball 34. Other substrate configurationsincluding cartridges, fibrous bundles, membranes or baffles may be used.The substrate may be formed from any material compatible with thesterile liquid, for example, plastic, metal or cellulosic material. Thesubstrate preferably is inert and non-degradable in the sterile liquid.The currently preferred materials for use as a substrate include glassor polymeric beads or pellets, fibers and non-woven materials such ascotton or cellulose, or metal foils. The preferred substrate will beantimicrobial, either naturally or will have attached thereto or coatedthereon an antimicrobial agent. Examples of naturally antimicrobialmaterials include certain metals, metal oxides, metal salts, metalcomplexes and metal alloys. For example, a metal foil such as silverfoil may be used. Substrates which are not anti-microbial haveimmobilized thereon an antimicrobial agent. Antimicrobial agents whichmay be used include the metallic and non-metallic agents discussed indetail herein.

The amount and/or type of the antimicrobial agent which is used in aparticular application will vary depending on several factors, includingthe amount of liquid which must be maintained in a sterile environment,the type and amount of contamination which is likely to occur, and thesize of the antimicrobial surface present in the dispenser or othercontainer. For example, certain metals, such as silver, are highlyeffective against most bacteria, but less effective against yeast (suchas Candida albicans). However, non-metallic agents such as biguanidecompounds, are toxic to yeast. Therefore, if the sterile liquid islikely to be exposed to contamination by both bacteria and yeast, acombination of silver and a biguanide compound can be used as theantimicrobial. The amount of antimicrobial used will be a minimum amountnecessary to maintain the sterility of the liquid. As stated above, thisamount will vary depending upon various considerations.

In a preferred embodiment, glass beads coated with metallic silver or asilver salt are added to the sterile liquid. The silver compound isattached to the beads such that the silver is substantiallynon-leachable. As used herein, the term "substantially "non-leachable"means that none or very small amounts (e.g., below a certain threshold)of the non-leachable material dissolves into the sterile liquid. It isunderstood that minute amounts of silver or other anti-microbial agentmay dissolve into the solution, but these amounts are minute. The amountof dissolved silver in the sterile liquid may be substantially reducedby passivating the silver coated surface by reaction with compoundswhich form silver salts or salt complexes. For example, reaction withhalogens, e.g., chlorine, iodine, bromine or mixtures thereof may beperformed, thereby generating a layer of silver halide on the metallicsilver coating. Other embodiments include, for example, pellets or beadsformed from an inert polymeric material, such as polyethersulfone,having an an organic, inorganic or a combination of the two types ofantimicrobial agents attached or coated on the surface. Preferredinorganic antimicrobial agents include elemental silver or silvercompounds. Preferred organic antimicrobial agents include cationic orpolycationic compounds, anionic or polyanionic compounds, non-ioniccompounds and zwitterionic compounds. Preferred compounds includebenzalkonium chloride derivatives and biguanide compounds, all of whichare discussed in detail hereinabove.

In methods of the invention the surfaces and pores of a filter or othersubstrate are coated with a metallic or non-metallic antimicrobialcompound, or a combination of the two types. In one embodiment, a filterhaving pores is provided, the filter is contacted with a carbonylcompound, the filter is dried, and the dried filter is contacted with ametal salt solution or metal carboxylate salt solution and anamine-containing compound solution so as to deposit the metal on thesurface and within a plurality of the pores. In one embodiment, thisfilter is then washed and dried. Other elements such as glass orpolymeric beads, glass wool, glass or polymeric fibers, membranes,cotton or other fibrous material or cartridges etc. can be treated inlike manner to coat or attach an antimicrobial agent.

The carbonyl compound includes, e.g., aldehydes, sugars, and aldehydefunctionality generating compounds. Aldehydes include compounds with theformula R(CHO)_(n), where R is any aliphatic, aromatic or heteroaromaticgroup and n is an integer greater or equal to 1. Examples of watersoluble aldehydes are glutaraldehyde, formaldehyde, acetaldehyde,butyraldehyde, glyceraldehyde, glyoxal, glyoxal disodium bisulfite,paraldehyde and cyclic trioxanes. Examples of water insoluble aldehydesare cinnamaldehyde and benzaldehyde. By sugar is meant a reducing sugar.Sugars include, e.g., fructose, glucose, lactose, maltose and galactose.By an aldehyde functionality generating compound it is meant a compoundcapable of generating aldehyde group(s). Examples of such compoundsinclude acetals and hemiacetals. Polymeric acetals, e.g.,paraformaldehyde and polyacetal, can also be used in this invention. Thecarbonyl compound acts as a reducing agent, so that the metal ion isreduced to the metal, e.g., silver ion is reduced to metallic silver.This electroless redox reaction occurs in situ in solution or in thesolid state. The carbonyl compound has affinity for aqueous andnon-aqueous phases and therefore can be used in the process of coatingeither hydrophilic or hydrophobic filters. If hydrophobic filters areused, the resulting metal coating confers hydrophilic properties on thecoated filter.

After treatment with the carbonyl compound, the filter or othersubstrate is either immersed directly into the metal salt solution ormetal carboxylate salt solution, or is dried first and then immersed inthis solution. Preferably, the filter is first dried. The drying stepincreases the metal coating within the pores of the filter and producesa more uniform and adhesive metal coating thickness on the surface andwithin the pores of the filter. Coating within the pores enhances thebacteriostatic or bacteriocidal properties of the filters.

Any metal which has bacteriostatic or bacteriocidal properties, asdescribed above, can be used in this invention to coat the substrate. Ina preferred embodiment, the metal is silver. The silver salts that canbe used in the metal coating process are salts that are capable ofsolubilizing, even to a limited degree, in aqueous media, to producesilver ions. Such salts include, e.g., silver nitrate, silver benzoate,silver tartrate and silver acetate, silver citrate or any silvercarboxylate.

Metal carboxylate salts include compounds with the formula R(COO⁻M⁺)_(n), where R is any aliphatic, aromatic or heteroaromatic group andn is an integer greater or equal to 1. Examples of metal carboxylatesalts include, e.g., silver, zinc, cadmium, mercury, antimony, gold,aluminum, copper, platinum and palladium salts of acetic, propanoic,lactic or benzoic acid; and mono- or di- sodium or potassium salts ofdiacids, e.g., oxalic, malonic, glutaric or tartaric acids. The termmetal carboxylate salts is also meant to include carboxylic acids whichare capable of forming carboxylate salts in situ under conditionsincluding the presence of a base and a metal ion, and compounds whichare capable of forming carboxylic or carboxylate groups in situ,including, e.g., esters, lactones, anhydrides and amides.

By amine-containing compound it is meant a compound capable of producinga metal-amine complex when metal salts react with amines under basicconditions. Examples of amine-containing compounds include ammoniumhydroxide, ammonia, and aliphatic, aromatic and heteroaromatic amines.

In another embodiment, a filter having pores is provided, the filter iscontacted with an activator, the filter is dried, and the dried filteris contacted with a metal salt or metal carboxylate salt solution so asto deposit the metal on the surface and within a plurality of the poresof the filter. The activator is a salt of a metal including, e.g., tin,titanium, vanadium, chromium, manganese, iron, cobalt, nickel, copper,zinc, germanium, selenium, zirconium, niobium, molybdenum, technetium,ruthenium, rhodium, palladium, antimony, tellurium and lead. A preferredactivator is tin dichloride. An alternative embodiment is to contact thedried filter with an amine containing compound in addition to the metalsalt or metal carboxylate salt solution.

Additionally, many types of metals can be plated onto the surface ofsuitably primed polymeric materials using standard well knownelectroplating techniques or by electroless methods. It is necessary toprime the polymer surface to allow for the electroplating process tooccur because most polymers are electrically insulating and do not carryan electrical current. Priming deposits a very small amount of metalonto the surface of the polymer allowing for the subsequent electrolyticdeposition of a metal from solution.

The metal coating on the filters derived from any of the methodsdiscussed above, can be further treated to produce a metal oxidecoating, as described in Example 8, or a metal halide coating, asdescribed in Example 16.

The invention further provides methods for attaching or coatingnon-metallic antimicrobial agents to a surface. In this embodimentantimicrobial agents including the anti-bacterial, anti-viral and/oranti-fungal agents described herein are non-leachably immobilized on thesurface of a filter or other element. These non-metallic agents may beused in lieu of or in addition to the metallic antimicrobial agents. Thenon-metallic agents may be immobilized by any suitable method, includingcovalent bonding, ionic attraction, coulombic interaction, hydrogenbonding and interpenetrating networks, for example. Methods forattaching organic antimicrobial agents to a metal surface are describedin Examples 16 and 17.

The invention further provides methods for attaching or coating acombination of a metallic and non-metallic antimicrobial agents to asurface. In this embodiment antimicrobial agents including theanti-bacterial, anti-viral and/or anti-fungal agents described hereinare non-leachably immobilized on the surface of a filter or otherelement. The non-metallic agents may be immobilized by any suitablemethod, including covalent bonding, ionic attraction, coulombicinteraction, hydrogen bonding, cross-linking (curing) andinterpenetrating networks, for example. The metallic antimicrobial maybe introduced in the non metallic antimicrobial coating either prior toor after applying the coating to the surface. The metallic antimicrobialmay consist of a metal, metal salt or metal complex may be introducedinto the non-metallic antimicrobial either as particles or as ahomogeneous solution. Methods for attaching organic antimicrobial agentsto a metal surface are described in Examples 16 and 17.

The substrate may be pretreated, if necessary, to activate the surface.In one embodiment, the surface is silylated to render it more receptiveto binding antimicrobial agents. Silylation can be carried out byart-recognized techniques including direct coupling reactions, graftingreactions and dendrimer-type reactions. The antimicrobial agent then isreacted with the resulting silyl functionality, or to a group attachedto the silyl functionality. Methods for immobilizing antimicrobialcompounds on silylated surfaces are described in Example 17.

In another embodiment, metallic and non-metallic antimicrobial agentsmay be attached to non-silylated surfaces. In this embodiment, thesurface is treated to obtain carboxylic or amine functionalities asdescribed above, and the antimicrobial agent is attached by reactionwith these functionalities. Methods for immobilizing antimicrobialmaterials on a non-silylated surface are described in Example 18.

This invention also includes the products made in accordance with thesemethods.

This invention also provides a method for dispensing sterile liquid byapplying pressure to the container of the liquid dispenser of thisinvention so as to discharge the sterile liquid from the container. Inone embodiment, the container has an elastically deformable wall,pressure is applied to deform the wall and force the sterile liquid fromthe container through the filter, and the wall is allowed to recover soas to draw gas from the surrounding atmosphere into the container, thegas being sterilized as it passes through the filter.

The liquid dispenser can be used for any purpose which requiresdispensing a sterile solution from a container. Such uses include, e.g.,medical related purposes, e.g., dispensing sterile liquids onto any partof the body of an organism or onto an object that is to be placed intothe body of an organism, e.g, for use in eye, ear, or nose care. Forexample, this invention provides a method for using the liquid dispenserof this invention for eyecare in an organism in which a sterile eyecareliquid is dispensed into an eye of the organism or onto an object thatis to be placed into the eye of the organism. Preferably, the sterileeyecare liquid is preservative-free. The sterile eyecare liquidincludes, e.g., liquid artificial tears, a solution for contact lenscare or a medicament. Examples of medicaments are antibiotics,decongestants, anti-inflammatories, anti-glaucoma agents, anti-bacterialagents, anti-viral agents, anesthetics, mydriatics, anti-cholingericsand miotics. An object that is to be placed into the eye includes, e.g.,a contact lens. Other uses include process filters for sterilization ofall types of solutions, e.g., drug solutions and instillation solutions;intravenous catheters, where a membrane unit is employed for theadmittance of air but prevents back flow of blood or other liquids;process filters for food products where sterility is required;dispensation of items such as baby formula where the presence of apreservative would be undesirable; and membrane filter units, e.g., forcampers and hikers where the generation of microbial free water isdesired without the possibility of future contamination.

EXAMPLES Example 1 Metal Vapor Deposition (MVD) of Silver Onto aPolyethersulfone Membrane

This example illustrates a method for depositing silver onto a surface,but not within the pores, of a membrane filter. A precutpolyethersulfone membrane (Supor 450, pore size 0.45 4M, hydrophilic,obtained from Gelman of Ann Arbor, Mich.) was mounted on a plate suchthat the surface to be coated faced the heating source of a metalevaporator. An approximately 4-6 inch long silver wire (obtained fromJohnson Matthey of Wardhill, Mass.) was rolled into a coil and placed onthe metal bridge in the evaporator. The evaporator was pumped down to10⁻⁵ Torr and a current of approximately 60-70 amperes was applied tomelt the silver. A uniform silver coating of the membrane surfaceresulted in about 15-30 secs. The current was turned off and theevaporator chamber was allowed to return to atmospheric pressure. Themembrane was turned over and the procedure repeated. The resultingmembrane had a uniform coating of silver on both surfaces, but notwithin the pores, as determined by scanning electron microscopy (SEM)and energy dispersive X-ray analysis (EDAX).

Example 2 Electroless Coating of Silver Onto a Polyethersulfone Membrane(Method 1)

This example illustrates a method for depositing metallic silver onto asurface, and within the pores, of a membrane filter. A polyethersulfonemembrane Gelman Supor 450, pore size 0.45 mM, hydrophilic) was precutinto a 47 mm disk. This membrane was immersed in 5 ml of glutaraldehyde(25% solution, obtained from Aldrich of Milwaukee, Wis.) for 1 min. at22° C., removed from the aldehyde solution and air dried thoroughly. Thetreated membrane was then immersed in 5 ml of the silver coatingsolution described in Example 7A, at pH approximately 12 (the pH canrange from approximately 8-14), at 35° C. for 15 secs. The platedmembrane was thoroughly rinsed with distilled water and dried in avacuum oven at 20° C. for 2 hrs. SEM coupled with EDAX showed uniformsilver coating on the membrane surface and within the pores.(Ag:S=0.4-0.5:1)

Example 3 Electroless Coating of Silver Onto a Polyethersulfone Membrane(Method 2)

This example illustrates a method for depositing metallic silver onto asurface and within the pores of a membrane filter. A polyethersulfonemembrane (Millipore, pore size 0.45 mM, hydrophobic, obtained fromMillipore Corp. of Bedford, Mass.), was precut into a 47 mm disk. Thismembrane was immersed in 5 ml of 0.1M a-D-glucose in an aqueous solutioncontaining 10% ethanol for 5 mins. at 22° C., removed from the sugarsolution and air dried thoroughly. The treated membrane was thenimmersed in 5 ml of the silver coating solution described in Example 7A,at pH approximately 12 at 35° C. for 2 mins. The plated membrane wasthoroughly rinsed with distilled water and dried in a vacuum oven at 20°C. for 2 hrs. SEM coupled with EDAX showed uniform silver coating on themembrane surface and within the pores. (Ag:S=0.8:1)

Example 4 Electroless Coating of Silver Onto a Polyethersulfone Membrane(Method 3)

A polyethersulfone membrane (Gelman, pore size 0.45 mM, hydrophilic) wasprecut in the form of a 47 mm disk. This was immersed in 5 ml of a 0.1Ma-D-glucose in an aqueous solution containing 10% ethanol for 5 minutesat 22 deg. C. It was then removed from the sugar solution and air driedthoroughly. The treated membrane was then immersed in 5 ml of a silverplating solution 1 (pH ˜12) at 35 deg. C. for 2 minutes. A rapiddeposition of metallic silver on the membrane surface ensued. The platedmembrane was thoroughly rinsed with distilled water and dried in avacuum oven at 20 deg. C. for 12 hours. SEM coupled with EDAX showeduniform silver coating on the membrane surface and within the pores.(Ag:S=0.3:1)

Example 5 Electroless Coating of Silver Onto a Polyethersulfone Membrane(Method 4)

This example illustrates a method for depositing metallic silver onto asurface and within the pores of a membrane filter. A polyethersulfonemembrane (Gelman Supor 450, pore size 0.45 mM, hydrophilic) was precutinto a 47 mm disk. This membrane was immersed in 5 ml of the silvercoating solution described in Example 7B, which was then heated to 55°C. and maintained at this temperature for 5 mins. Rapid deposition ofmetallic silver onto the membrane ensued. The plated membrane wasthoroughly rinsed with distilled water and dried in a vacuum oven at 20°C. for 12 hrs. SEM coupled with EDAX showed uniform silver coating onthe membrane surface and within the pores. (Ag:S=0.05:1)

Example 6 Electroless Coating of Silver Onto a Polyethersulfonemembrane: (Method 5)

This example illustrates a method for depositing metallic silver onto asurface and within the pores of a membrane filter. A polyethersulfonemembrane (Gelman Supor 450, pore size 0.45 mM, hydrophilic) was precutinto a 47 mm disk. This membrane was immersed in 5 ml of a solutioncontaining 1 g tin (II) chloride dihydrate (Aldrich), 1 ml concentratedHCl and 9 ml distilled water, at room temperature for 5 mins. Themembrane was dried and immersed in the silver coating solution,described in Example 7B, which was then heated to 55° C. and maintainedat this temperature for 3 mins. Rapid deposition of metallic silver onthe membrane surface ensued. The plated membrane was thoroughly rinsedwith distilled water and dried in a vacuum oven at 20° C. SEM coupledwith EDAX showed uniform silver coating on the membrane surface andwithin the pores. (Ag:S=0.3:1)

Salts of other metals including titanium, vanadium, chromium, manganese,iron, cobalt, nickel, copper, zinc, germanium, selenium, zirconium,niobium, molybdenum, technetium, ruthenium, rhodium, palladium,antimony, tellurium and lead may be used as activators in place of tindichloride prior to silver plating.

Example 7 Preparation of Silver Coating Solutions

(A) This example illustrates the preparation of the silver coatingsolution that is used in Examples 2, 3, 4 and 10. 3 ml of a silvernitrate solution (10 g silver nitrate dissolved in 10 ml distilledwater) was added to 3 ml of a sodium hydroxide solution (10 g sodiumhydroxide dissolved in 10 ml distilled water) at 22° C. A brownprecipitate of silver oxide formed rapidly. Concentrated ammoniumhydroxide (28% ammonia, obtained from EM Science of Gibbstown, N.J.) wasadded dropwise to the solution until the silver oxide dissolvedcompletely to give a clear solution of a soluble silver amine complexwith a pH of 12.

(B) This example illustrates the preparation of the silver coatingsolution that is used in Examples 5 and 6. Three (3) ml of a sodiumtartrate solution (tartaric acid disodium salt dehydrate, obtained fromAldrich) dissolved in 20 ml distilled water) was added to 3 ml of asilver nitrate solution (silver nitrate dissolved in 10 ml distilledwater) at 22° C. A white precipitate of silver tartrate formed rapidly.Concentrated ammonium hydroxide, 28% ammonia (obtained from EM Science),was added dropwise to the solution until the silver tartrate dissolvedcompletely to give a clear solution of ammoniacal silver tartarate witha pH of approximately 12.

Example 8 Oxygen Plasma Treatment of Silver Coated Membrane Filters

This example illustrates a method for treating a silver coated membranefilter with oxygen plasma so as to produce a silver oxide coating. Asilver coated polyethersulfone membrane, obtained from either Example 1or 2, was mounted on a glass holder and placed inside the reactionchamber of a plasma reactor so that both surfaces of the filter wereexposed to the plasma. The reaction chamber was purged with oxygen threetimes. The pressure of the chamber was adjusted to 300 mTorr, the powermaintained at 100 watts, and the membrane subjected to oxygen plasma for2 mins.

Example 9 Surface Modification of Silver Coated Membrane Filters

This example illustrates a method for treating a silver coated membranefilter with a second compound that has anti-bacterial or anti-viralproperties, 3.44 mg (20 mmol) of benzalkoniumchloride thiol (BAC-S),obtained as described in Example 9, was dissolved in 5 ml of absoluteethanol that was degassed for 1 hr under dry nitrogen. A freshly silvercoated polyethersulfone membrane obtained from Example 1 or 2, wasimmersed in this solution at 22° C. for 16 hrs, rinsed in absoluteethanol, and dried under a stream of nitrogen.

Example 10 Synthesis of Benzalkoniumchloride Thiol (BAC-S)

This example illustrates a method for the synthesis of BAC-S from10-chlorodecanethiol which in turn is synthesized fromw-chlorodecane-thioacetate.

(a) ω-Chlorodecanethioacetate

Triphenylphosphine (6.53 g, 25 mmol) (obtained from Aldrich) wasdissolved in 95 ml of dry, distilled tetrahydrofuran and the solutionwas cooled to 0° C. under dry nitrogen. 4.9 ml (25 mmol) ofdi-isopropylazodicarbonate (obtained from Aldrich) was added to thesolution. The reaction mixture was stirred for 30 min. at 0° C. duringwhich time a white precipitate formed. A 1M solution of10-chloro-1-decanol (4.82 g in 25 ml THF (tetrahydrofuran) (obtainedfrom VWR Scientific of Boston, Mass.) was added. 2.3 g of thiolaceticacid (obtained from Aldrich) in 20 ml THF was subsequently added. Theresulting clear solution was stirred at 0° C. for 15 mins and warmed toroom temperature. Two drops of water were added. The solvent was removedunder reduced pressure, the residue was dissolved with ethyl ether andcrystals of triphenylphosphine oxide were removed by filtration.Evaporation of the ether resulted in the crude product,ω-chlorodecanethioacetate, as a yellow oil. This product was distilledunder reduced pressure (92°-95° C./10 mM) to give the pure compound as apale yellow oil (3.23 g, 57%). IR neat, KBr plates cM-1: 1728,s,(O═C--S), TLC, silica (hexane:dichloromethane, 60:40 eluent) Rf=0.7.

(b) 10-Chlorodecanethiol

ω-Chlorodecanethioacetate (3.2 g, 1.2 mmol) was hydrolyzed in 30 ml ofmethanol, that was degassed under dry nitrogen for 4 hrs, containing 1.6g (1.2 mmol) anhydrous potassium carbonate (obtained from Aldrich) at22° C. The suspension was stirred for 1 hr, and then quenched with 0.75ml glacial acetic acid (obtained from VWR Scientific). The potassiumcarbonate was filtered and the solvent removed under reduced pressure toyield 10-chlorodecanethiol as a pale yellow oil, 2.4 g, 1.1 mmol). TLC,silica (hexane:dichloromethane, 60:40 eluent) Rf═O

(c) Benzalkoniumchloride Thiol (BAC-S)

2.4 g (11 mmol) of 10-chlorodecanethiol was reacted with 1.8 g (14 mmol)of N,N-dimethylbenzylamine (obtained from Aldrich) in 50 ml of dry THF.The reaction mixture was refluxed for 20 hrs and cooled. White crystalsof BAC-S separated out. These crystals were filtered, washed with THFand dried, yielding 0.85 g of product.

Example 11 Synthesis of Alkane Thiol Derivative of PolyhexamethyleneBiguanide (PHMB-S) and Chain Extended Polyhexamethylene Biguanide(PHMBCE-S)

This example illustrates a method of the synthesis of alkane thiolderivative of polyhexamethylene biguanide (PHMB-S) and chain extendedpolyhexamethylene biguanide (PHMBCE-S):

(a) Neutralization of Polyhexamethylene biguanide-bis hydrochloride

Polyhexamethylene biguanide-bis-hydrochloride (Zeneca, Wilmington,Del.), 1 g, was neutralized by addition of 2 ml. of a 10% NaOH solution.The solvent was evaporated and the residual solid was washed rapidlywith water to minimize dissolution and dried to give polyhexamethylenebiguanide (PHMB).

(b) Synthesis of Chain extension of chain polyhexamethylene biguanide(PHMBCE)

Polyhexamethylene biguanide (PHMB) was reacted withethyleneglycol-bis-glycidylether (Aldrich) in a 1:0.9 mole ratiorespectively in anhydrous DMSO at 50 deg.C. for 12 hours. The solventwas evaporated under reduced pressure and the solid residue was washedwith ether and dried.

(c) Synthesis of alkanethiol derivative of polyhexamethylene biguanide(PHMB-S) and chain extended polyhexamethylene biguanide (PHMBCE-S)

Polyhexamethylene biguanide (PHMB-S) and chain extendedpolyhexamethylene biguanide (PHMBCE-S) respectively 1 mole equivalent)were reacted with 10-chlorodecanethiol (0.5 mole equivalent) describedin Example 10b in anhydrous DMSO at 50 deg.C for 12 hours. The solventwas evaporated under reduced pressure and the solid residue was washedwith ether and dried to yield the title compounds.

Example 12 Electroless Coating of Silver Onto Tubing For Use in NozzleAssembly

This example illustrates a method for depositing silver onto the innersurface of tubing which can be used for the passageway walls in thenozzle assembly of the liquid dispenser. Polyethylene tubing (2 incheslong, 800 mM ID) (obtained from Putnam Plastics Corp. of Dayville,Conn.) was immersed in a 25% aq. glutaraldehyde solution andultrasonicated at 20° C. for 2 mins. The tubing was then driedthoroughly and silver coating solution, as described in Example 7A, wasdrawn into the tubing with a pipette. The plating solution was allowedto soak inside the tubing for 3 mins at 20° C., the excess solution wasthen expelled and the inside of the tubing was flushed with distilledwater. A uniform silver coating resulted on the inside surface of thetubing.

The tubing was tested for bacteriocidal activity. Control or silvertreated plastic tubing was inoculated with 10 mls of a suspension ofPseudomonas dimunata containing 5×10⁷ organisms. The tubes containingbacterial suspension were incubated 15 hours at 37° C., at which timethe tubes were placed in thioglycollate bacterial culture medium (1 cc)and vortexed. Aliquots of this solution were removed and seriallydiluted and 100 mls of these dilutions were plated onto NZY agar plates.The plates were incubated overnight at 37° C. and the bacterialconcentrations were determined by counting bacterial colonies.

Example 13 Surface Modification of Silver Plated Membrane to GiveHydrophobic/Hydrophilic Surface

Method A

This example illustrates a method for producing a silver coated membranefilter that is partially hydrophobic and partially hydrophilic. 20.2 mg(20 mmol) of 1-dodecanethiol (obtained from Sigma Chemical Co., St.Louis, Mo.) was dissolved in 5 ml of absolute ethanol that was degassedfor 1 hr under dry nitrogen. A freshly silver coated (by MVD orelectroless process) polyethersulfone membrane (Gelman Supor 400, poresize 0.45 mM, hydrophilic) was partially immersed in this solution at22° C. for 16 hours. The membrane was then rinsed in absolute ethanoland dried under a stream of nitrogen. The resulting surface treatedmembrane was hydrophobic (non-wetting) in the area treated with thealkyl thiol while remaining hydrophilic in the non-treated area.

Method B

This example illustrates a method for producing a partially hydrophobicarea in a hydrophilic membrane (with or without a silver coating).

The hydrophilic membrane was immersed in a 2% n-hexane solutioncontaining a 1:1:1 mixture (by weight) of methyltriacetoxy silane,ethyltriacetoxysilane and silanol terminated polytrimethylsiloxane(obtained from Huls America, Piscataway, N.J.). The membrane was airdried at room temperature for 30 min., after which it was heated at 120°C. for 30 min. to render the treated area totally hydrophobic.

Example 14 Silver Coating of Polymethylmethacrylate (PMMA) Sheets

This example illustrates methods for depositing metallic silver onto thesurface of PMMA sheets.

Method A

A commercially obtained PMMA sheet was cut in the form of a slide andthe surface was cleaned by ultrasonication in absolute ethanol for 1minute at room temperature. The cleaned slide was then immersed in 30 mlof an activator solution consisting of 10% tin dichloride dihydrate(SnCl₂ 2H₂ O, obtained from Allied Chemicals of New York, N.Y.), 45%absolute ethanol and 45% distilled water and ultrasonicated at 45° C.for 15 minutes. The slide was rinsed several times with distilled water.It was then immersed in a 10% aqueous solution of silver nitrate for 15minutes at room temperature. During this time the slide acquired a browntinge due to the deposition of silver. The coated slide was then rinsedwith distilled water and dried. The silver coating was adherent to thepolymer and did not cause loss of transparency of the slide.

Method B

A PMMA slide was treated with plasma as described in Example 8, followedby immersion into a 2% aqueous solution of polyethyleneimine (PEI)(Aldrich Chemical Co., Milwaukee, Wis.) for 15 min. The slide was rinsedwith water, after which it was first immersed in a 5% aqueousglutaraldehyde solution (Aldrich) for 5 min., followed by a 10% silvernitrate solution for 5 min. The surface was then subjected toelectroless silver plating in a silver tartarate solution as describedin Example 7B to give a uniform, adhesive silver mirror.

Example 15 Silver Coating of Polypropylene (PP)

This example illustrates a method for depositing a metallic silvercoating onto the surface of polypropylene slides (obtained from EastmanChemical Product, Inc. Kingsport, Tenn.). The slides were immersed in 30ml of a 5% solution of chlorosulfonic acid (obtained from Aldrich ofMilwaukee, Wis.) in chloroform at 50° C. for 5 mins. They were allowedto dry for 15 mins. then immersed in a 1M aqueous solution of sodiumhydroxide after which they were rinsed thoroughly with distilled water.The surface treated slides were subjected to the silver coatingprocedure described in Example 4 using the plating solution described inExample 7B. This resulted in a uniform silver coating that is adherentto the polypropylene surface.

Example 16 Modification of Metallic Surfaces with AntimicrobialCompounds Generation of Adherent Silver Halide Surfaces

Method A

Freshly silver plated membranes (by MVD or electroless method) wereexposed to either vapors of chlorine gas (generated in situ by reactionof sodium hypochlorite with concentrated HCl) or to bromine vapors for0.5 to 1 min. at room temperature. The metallic surface was rapidlycoated with a layer of silver chloride and silver bromide respectively.The membranes were then washed with water and dried.

Method B

Freshly silver plated membranes (by MVD or electroless method) wereimmersed in (i) an aqueous solution of 0.9% NaCl, or (ii) a 2% aqueoussolution of bromine, or (iii) a 2% aqueous solution consisting ofequimolar amounts of bromine, iodine and potassium iodide at roomtemperature for 15 minutes. A rapid coating of silver halide (orhalides) resulted on the surface of the metallic coating. The membraneswere then washed with water and dried.

Method C

Freshly silver plated membranes (by MVD or electroless method) wereimmersed in a 2% aqueous solution consisting of equimolar amounts ofiodine and potassium iodide at room temperature for 15 minutes. A rapidcoating of silver iodide resulted on the surface of the metalliccoating. The membranes were then washed with ethanol followed by waterand dried.

Modification of silver plated membrane with antimicrobial

(a) with benzalkoniumchloridethiol (BAC-S): 3.44 mg (10⁻³ mmol) ofbenzalkoniumchloridethiol (BAC-S) was dissolved in 5 ml of absoluteethanol that was degassed for 1 hr under dry nitrogen. A freshly silvercoated (by MVD or electroless process) polyethersulfone membrane (GelmanSupor 450, pore size 0.45 mM, hydrophilic) was immersed in the resultingsolution at 22 deg. C. for 16 hours. The membrane was then rinsed inabsolute ethanol and dried under a stream of nitrogen.

(b) with alkanethiol derivative of polyhexamethylene biguanide(PHMBCE-S): 3 mg of polyhexamethylenebiguanide (PHMB-S) and chainextended polyhexamethylenebiguanide (PHMBCE-S) respectively weredissolved in 5 ml of degassed water. A freshly silver coated (by MVD orelectroless process) polyethersulfone membrane (Gelman Supor 450, poresize 0.45 uM, hydrophilic) was immersed in the resulting solution at 22deg. C. for 16 hours. The membrane was then rinsed with water, 1% HClsolution followed by water and dried.

Modification of gold foil with antimicrobial

Commercially obtained gold foil was treated with oxygen plasma for 3minutes to clean the surface and improve wetability. The foil was thenimmersed in the resulting solution at 22 deg. for 16 hours. The membranewas then rinsed in absolute ethanol and dried under a stream nitrogen.

Modification of silver and gold surfaces with biguanides

Freshly silver plated membranes and gold foil (cleaned as describedabove) were immersed in aqueous solutions of diamine terminatedpolyhexamethylenebiguanide (ICI Zeneca) its chain extended analog(synthesized) that were suitably modified to incorporate a thiol groupat 22 deg. C. for 16 hours. The membrane was then rinsed with waterfollowed by a 1% HCl solution. After rinsing thoroughly with water theywere dried.

Example 17 Modification of Silver halide surfaces with biguanides

Membranes coated with silver halides were immersed in either one of thecoating solutions (Examples 18a or 18b) for 1 minute, excess solutionremoved from the membrane surface, after which they were allowed to dryat ambient temperature for 0.5 hour. The membrane was then cured at 130°C. for 30 minutes. It was then extracted with a 50% aqueous ethanolsolution for 30 minutes at 80° C., followed by 3 times with water (1hour each, 80° C.) after which they were air dried for 0.5 hour at 80°C. The coated membranes were then immersed in a solution containing 0.5%of silver iodide and 12.5% (wt/vol.) potassium iodide in 100 mL of 50%aqueous ethanol at ambient temperature for 5 minutes. The membranes wererinsed with 50% aqueous ethanol followed by water extraction (3×100 mL,1 hour each at 80° C. They were then air dried.

Example 18 Preparation of biguanide coating solutions

Polyhexamethylenebiguanide (PHMB) was precipitated from a 20% aqueoussolution of polyhexamethylene biguanidedihydrochloride ((ZenecaBiocides, Wilmington, Del.) by addition of two volume equivalents of 5%aqueous NaOH. The resulting precipitate was separated from the alkalinesolution, dissolved in anhydrous DMF and reprecipitated withacetonitrile. The precipitate was filtered and dried under vacuum at 50°C. for 6 hours.

(a) 2 mL of a 5% (weight/volume) PHMB solution in anhydrous ethanol wasadded to 2 mL of 5% (weight/volume) solution of4,4'-methylene-bis(N,N-diglycidylaniline) (Aldrich Chemical Company,Milwaukee, Wis.) dissolved in a 4:1 (vol/vol) ethanol/acetonitrilemixture. The solution was stirred at room temperature for 20 minutes anddiluted with 76 mL of anhydrous ethanol to a solution containing 0.25%solids.

(b) 2 mL of a 5% (weight/volume) PHMB solution in anhydrous ethanol wasadded to 2 mL of 5% (weight/volume) solution of4,4'-methylene-bis(N,N-diglycidylaniline) (Aldrich Chemical Company,Milwaukee, Wis.) dissolved in a 4:1 (vol/vol) ethanol/acetonitrilemixture. The solution was refluxed at 90°-95° C. for 15 minutes. Thesolution was cooled and diluted with 76 mL of anhydrous ethanol to asolution containing 0.25% solids.

(c) 2 mL of a 5% (weight/volume) PHMB solution in anhydrous ethanol wasadded to 2 mL of 5% (weight/volume) solution of4,4'-methylene-bis(N,N-diglycidylaniline) (Aldrich Chemical Company,Milwaukee, Wis.) dissolved in a 4:1 (vol/vol) ethanol/acetonitrilemixture. The solution was refluxed at 90°-95° C. for 15 minutes. Thesolution was cooled diluted with 16 mL of anhydrous DMF. 50 mg of finelyground silver iodide was dispersed in the diluted solution and theresulting suspension was heated to 80° C. with stirring to produce aclear solution. This was cooled and filtered to give a clear,homogeneous solution

(d) 2 mL of a 5% (weight/volume) PHMB solution in anhydrous ethanol wasadded to 2 mL of 5% (weight/volume) solution of4,4'-methylene-bis(N,N-diglycidylaniline) (Aldrich Chemical Company,Milwaukee, Wis.) dissolved in a 4:1 (vol/vol) ethanol/acetonitrilemixture. The solution was refluxed at 90°-95° C. for 15 minutes. Thesolution was cooled diluted with 16 mL of anhydrous DMF. 50 mg of finelyground silver iodide was dispersed in the diluted solution to give awell dispersed suspension.

Example 19 Introduction of Antimicrobial Compounds on Silylated Surfaces

The following reaction schemes demonstrate methods for preparing asilylated surface and reacting an antimicrobial agent to the silylfunctionality. ##STR2##

Example 20 Introduction of Antimicrobial Compounds on Non-SilylatedSurfaces Incorporation of silver salts on PMMA surfaces

I) Polyacrylic acid was introduced on the surface of PMMA by graftpolymerization of acrylic acid to obtain surface functionalization. Basetreatment followed by an aqueous silver nitrate solution resulted in theformation of silver salt of polyacrylic acid

II) The polyacrylic acid coating obtained on PMMA by the above methodwas further modified by coupling the carboxylic groups of the acrylicacid with cysteine using DCC. The membrane was then treated with basefollowed by an aqueous silver nitrate solution to give a mixture ofsilver carboxylate and silver thiolate.

Attachment of antimicrobials to coulombic multilayers onactivated-polymeric surfaces

Polymeric surfaces activated by (i) oxidative chemical procedures, (ii)plasma or (iii) e-beam are then treated sequentially withpolyethyleneimine (PEI) and polyacrylic acid. This process may berepeated over several cycles to obtain the desired amplification.Antimicrobial compounds (or suitably modified derivatives thereof) arethen covalently attached to the amine functionalities in PEI. ##STR3##

Coating of antimicrobials to activated polymeric surfaces

Polymeric surfaces are activated by (i) oxidative chemical methods or(ii) plasma. Solutions of antimicrobial compounds (or suitably modifiedderivatives thereof) are then applied on the surfaces and cured(thermally, photochemically or chemically) to result in stable, nonleachable coatings

Example 21 Antimicrobial Coating of Polymeric Surface

(a) Polypropylene coupons (10×10 cm) were surface oxidized by knownmethods (chemical or plasma). The coupons were immersed in the coatingsolution described in Example 18a or 18b for 1 minute, excess solutionremoved from the membrane surface, after which it was allowed to dry atambient temperature for 0.5 hour. The coupons were then cured at 130° C.for 30 minutes. They were then extracted with a 50% aqueous ethanolsolution for 30 minutes at 80° C., followed by 3 times with water (1hour each, 80° C.) after which they were air dried for 0.5 hr. at 80° C.The coated membranes were then immersed in a solution containing 0.5% ofsilver iodide and 12.5% (wt/vol.) potassium iodide in 100 mL of 50%aqueous ethanol at ambient temperature for 5 minutes. The membranes wererinsed with 50% aqueous ethanol followed by water extraction (3×100 mL,1 hour each at 80° C. They were then air dried.

(b) Polypropylene coupons (10×10 cm) were surface oxidized by knownmethods (chemical or plasma). The coupons were immersed in the coatingsolution described in Examples 18c or 18d for 1 minute, excess solutionremoved from the surface, after which it was allowed to dry at ambienttemperature for 0.5 hour. The coupons were then cured at 130° C. for 30minutes. They were then extracted with a 50% aqueous ethanol solutionfor 30 minutes at 80° C., followed by 3 times with water (1 hour each,80° C.) after which they were air dried for 0.5 hr. at 80° C.

Example 22 Preparation of surface coating solutions

(a) 2 mL of a 5% (weight/volume) PHMB solution in anhydrous ethanol wasadded to 2 mL of 5% (weight/volume) solution of4,4'-methylene-bis(N,N-diglycidylaniline) (Aldrich Chemical Company,Milwaukee, Wis.) dissolved in a 4:1 (vol/vol) ethanol/acetonitrilemixture. The solution was refluxed at 90°-95° C. for 15 minutes. Thesolution was cooled diluted with 16 mL of anhydrous DMF. 50 mg of finelyground silver iodide was dispersed in the diluted solution and theresulting suspension was heated to 80° C. with stirring to produce aclear solution. This was cooled and filtered to give a clear,homogeneous solution.

(b) 2 mL of a 5% (weight/volume) PHMB solution in anhydrous ethanol wasadded to 2 mL of 5% (weight/volume) solution of4,4'-methylene-bis(N,N-diglycidylaniline) (Aldrich Chemical Company,Milwaukee, Wis.) dissolved in a 4:1 (vol/vol) ethanol/acetonitrilemixture. The solution was refluxed at 90°-95° C. for 15 minutes. Thesolution was cooled diluted with 16 mL of anhydrous DMF. 50 mg of finelyground silver iodide was dispersed in the diluted solution to give awell dispersed suspension.

Example 23 Anti-bacterial Properties of Differently Treated MembraneFilters

This example illustrates a method for testing different types ofmembrane filters with different pore sizes, which have been coated withdifferent compounds by different procedures, for theirbacteriocidal/bacteriostatic properties.

Filters, either control or treated, were placed into Gelman plasticfilter holders and the entire unit was autoclaved 30 minutes at 121RC.Using asceptic techniques under a laminar flow hood, sterile bacterialmedia, sterile saline solution or a preservative free artificial tearsolution ("Tears Plus," obtained from Johnson and Johnson) wasintroduced to eliminate air pockets and to ensure proper flow throughthe assembled filter apparatus. The challenge organism, eitherPseudomonas dimunata, Candida albicans or a "cocktail" consisting of (i)Pseudomonas dimunata, Bacillus subtilis, and Escherichia coli, or (ii)Staphylococcus aureus and Pseudomonas aeruginosa at a concentration of10⁷ organisms per ml, was purged through the filter using a 3 cc syringeand manual pressure. Approximately 1 mL of liquid was expelled first andwas checked for sterility so to ensure that the membrane was properlysealed. Three drops of eluate were collected and tested for sterility.The outlet tip was maintained in a sterile environment using a cleansterile cover and the entire unit was stored at 37° C. for the course ofthe experiment. The sterility of the eluate was tested daily bycollecting three drops (approximately 150 ml) of bacteria/media inoculuminto sterile thioglycollate medium which was then placed in a 37° C.shaker overnight and assessed the next day for sterility. As themedium/bacteria inoculum level lowered over the course of theexperiment, the input syringe was removed and fresh sterile media wasadded and reattached to the filter holder unit. A filter was consideredto have "failed" when the sterility check failed on two consecutivetests, the failed filter apparatus still held air pressure under water,and the failed sterility check demonstrated by gram stain the expectedmorphology of the test organism.

Table I summarizes the results. "GS" is Gelman Supor 400 membranes; "GH"is Gelman HT650 membranes; "M" is Millipore membranes. The type ofmaterial that the membranes are composed of is indicated by "PES" forpolyethersulfone and "PVDF" for polyvinylidene fluoride. "mM" indicatesthe micron pore size of the membrane. "-" indicates that the membranewas untreated. "MVDAG" indicates the membrane was treated to produce asilver coating by the metal vapor deposition method as described inExample 1. "TAg" indicates the membrane was treated to produce a silvercoating by the electroless method of this invention as described inExample 2, Method 1. "+P" indicates the silver coated membrane wastreated with oxygen plasma to produce a silver oxide coating asdescribed Example 7. "+B" indicates that the silver coated membrane wastreated with BAC-S (benzalkoniumchloride thiol) to produce a layer ofBAC-S over the silver coating as described in Example 8. "+BG" indicatesthat the silver coated membrane was treated with PHMB-S or PHMBCE-S."+Cl" indicates that the membrane was treated with chlorine or NaCl."+Br" indicates that the membrane was treated with bromine. "+Br+I"indicates that the membrane was treated with a mixture of bromine andiodine. "+I" indicates that the membrane was treated with iodine."+I+PHMB" indicates that the silver iodide coated membrane additionallywith a PHMB coating. "+PHMB" indicates that the membrane was coated withPHMB. "+PHMB+I" indicates that the membrane was coated with PHMBfollowed by silver iodide introduction.

The numbers in the "Days" column indicate the average number of daysuntil the filter failed, as determined by the criteria discussed above.A ">#" indicates that no failure was detected for the duration of thetest, i.e., the number of days indicated.

The various growth media used are indicated by "B" for sterile bacterialmedia, "S" for sterile saline solution, and "T" for "Hypo Tears." apreservative free artificial tear solution by Johnson & Johnson.Sterilization of the membranes was achieved either by autoclaving at121RC for 30 minutes ("A"), or placement in absolute ethanol for 20minutes ("Et"). The "No." indicates the number of samples of membranesthat were tested

                  TABLE 1    ______________________________________    Bacterial Challenge Experiments on Silver Coated Membranes                                      Growth                                            Steril-    Mfgr Type    mm     Treatment                                 Days Medium                                            zation                                                  No.    ______________________________________    GS   PES     0.45   --       7    B     A     3    GS   PES     0.45   MVDAG + P                                 10   B     A     3    GS   PES     0.45   TAg      22   B     A     3    GS   PES     0.45   TAG + P  16   B     A     3    GS   PES     0.45   MVDAG + B                                 14   B     A     3    GS   PES     0.45   TAG + B  >77  B     A     3    GS   PES     0.45            7    B     A     9    GS   PES     0.45   TAg      >25  B     A     6    GS   PES     0.45            10   T     A     9    GS   PES     0.45   TAg      >175 T     A     9    GS   PES     0.45            3    S     A     6    GS   PES     0.45   TAg      >57  S     A     6    GH   PES     0.65            4    T     A     3    GH   PES     0.65   TAg      >82  T     A     3    GS   PES     0.65            1    S     A     5    GS   PES     0.65   TAg      >145 S     A     5    m    PVDF    0.22            5    B     Et    1    m    PVDF    0.22   MVDAG    >14  B     Et    1    m    PVDF    0.22   MVDAG + P                                 >14  B     Et    1    m    PVDF    0.22   TAg      >14  B     Et    1    m    PVDF    0.22   TAg + p  >14  B     Et    1    m    PVDF    0.22   TAG + B  >14  B     Et    1    m    PVDF    0.22            15   B     A     3    m    PVDF    0.22   TAg      >170 B     A     3    GS   PBS     0.45   TAg + B  >71  S     A     3    GS   PES     0.45   THE + I +                                 >97  S     Et    3                        PHMB    GS   PES     0.65   TAG + B  >71  S     A     3    GS   PES     0.65   TAG + BG >71  S     A     3    GS   PES     0.65   TAG + Cl >62  S     A     3    GS   PES     0.65   TAG + Br >36  S     A     2                                                  5    GS   PES     0.65   TAG + Br + I                                 >20  S     A     1                                                  5    m    PVDF    0.45            2    B     Et    1    m    PVDF    0.45   MVDAG    5    B     Et    1    m    PVDF    0.45   MVDAG + P                                 >11  B     Et    1    m    PVDF    0.45   TAg      >11  B     Et    1    m    PVDF    0.45            7    B     A     3    m    PVDF    0.45   TAg      >70  B     A     3    ______________________________________

Example 24 Anti-Microbial Properties of Differently Treated Substances

This example illustrates the anti-microbial effect of various substrateshaving immobilized thereon anti-microbial agents. The substrates includeglass beads, polyethersulfone (PES) pellets, gold foil and PESmembranes. The test was carried out as described in Example 20. Theresults are shown in Table 2.

                                      TABLE 2    __________________________________________________________________________    TYPE      MEDIUM                   ORGANISM                         COATING    COUNT 1(24 hrs)                                            NUMBER    __________________________________________________________________________    1 Tear Soln (P)*                   PD               0    2 Tear Soln (PF)*                   PD               6.2 × 10.sup.3    3 Glass Beads              A    PD    Uncoated   1.0 × 10.sup.4                                            7    4 Glass Beads              A    PD    Silver coated                                    63      7    5 Glass Beads              A    PD    Uncoated   2.3 × 10.sup.3                                            2    6 Glass Beads              A    PD    Silver coated                                    33      2    7 PES Pellets              A    PD    Uncoated   4.0 × 10.sup.3                                            25    8 PES Pellets              A    PD    Silver coated                                    0       25    9 PES Pellets              A    PD    Uncoated   2.0 × 10.sup.3                                            5    10 PES Pellets              A    PD    Silver coated                                    2       5    11 Tear Soln (P)*              A    BC1              0    12 Tear Soln (PF)*              A    BC1              3.5 × 10.sup.6    13 PES Pellets              A    BC1   Uncoated   3.5 × 10.sup.6                                            25    14 PES Pellets              A    BC1   Silver coated                                    0       25    15 PES Pellets              A    BC1   Silver + Add. Coat..sup.1                                    0       25    16 PES Pellets              B    BC1   Uncoated   8.0 × 10.sup.6                                            25 (New beads)    17 PES Pellets              B    BC1   Silver coated                                    0       25 (New beads)    18 PES Pellets              B    BC1   Uncoated   8.0 × 10.sup.6                                            25 (Used beads**)    19 PES Pellets              B    BC1   Silver + Add. Coat..sup.1                                    0       25 (Used beads**)    20 PES Pellets              B    BC1   Silver + Add. Coat..sup.2                                    0       25 (Used beads**)    21 Tear Soln (P)*              A    CA               0    22 Tear Soln (PF)*              A    CA               2.5 × 10.sup.4    23 PBS Control              B    CA               8.4 × 10.sup.4    24 PES Pellets              B    CA    Uncoated   1.5 × 10.sup.5                                            25    25 PES Pellets              B    CA    Silver + Add. Coat..sup.2                                    10      25    26 PES Pellets              B    CA    Silver + Add. Coat..sup.3                                    3.5 × 10.sup.2                                            25    27 Gold Foil              B    BC1   Uncoated   9.6 × 10.sup.5                                            2.5 × 1.25.sup.+    28 Gold Foil              B    BC1   Silver + Add. Coat..sup.3                                    0       2.5 × 1.25.sup.+    29 Gold Foil              B    BC1   Silver + Add. Coat..sup.3                                    0       2.5 × 1.25.sup.+    30 Gold Foil              B    CA    Uncoated   7.4 × 10.sup.3                                            2.5 × 1.25.sup.+    31 Gold Foil              B    CA    Silver + Add. Coat..sup.2                                    1.6 × 10.sup.3                                            2.5 × 1.25.sup.+    32 Gold Foil              B    CA    Silver + Add. Coat..sup.3                                    0       2.5 × 1.25.sup.+    33 Polypropylene (PP)              B    BC1              1 × 10.sup.6                                            2.5 × 1.25.sup.+    34 Polypropylene (PP)              B    BC1   Silver salt + Add. Coat..sup.4                                    0       2.5 × 1.25.sup.+    35 Polypropylene (PP)              B    BC2   Silver salt + Add. Coat..sup.4                                    0       2.5 × 1.25.sup.+    36 Polypropylene (PP)              B    PSA   Silver salt + Add. Coat..sup.4                                    0       2.5 × 1.25.sup.+    37 Polypropylene (PP)              B    BC1   Silver salt + Add. Coat..sup.5                                    0       2.5 × 1.25.sup.+    38 Polypropylene (PP)              B    BC2   Silver salt + Add. Coat..sup.5                                    0       2.5 × 1.25.sup.+    39 Polypropylene (PP)              B    BC1   Silver salt + Add. Coat..sup.6                                    0       2.5 × 1.25.sup.+    40 Polypropylene (PP)              B    BC2   Silver salt + Add. Coat..sup.6                                    0       2.5 × 1.25.sup.+    41 Polypropylene (PP)              B    PSA   Silver salt + Add. Coat..sup.6                                    0       2.5 × 1.25.sup.+    Bacterial Challenge on 0.65 uM (Gelman PES, MT 650) membranes    42 PBS Control BC2              2.5 × 10.sup.6    43 0.65 uM PES              B    BC2   silver     0       13 mm.sup.++    44 0.65 uM PES              B    BC2   Silver salt                                    0       13 mm.sup.++    1/30 Bacterial Challenge on 0.45 uM (Gelman PES, Super 450) membranes    45 0.45 uM PES              B    BC1              1 × 10.sup.6                                            13 mm.sup.++    46 0.45 uM PES              B    BC2              1 × 10.sup.6                                            13 mm.sup.++    47 0.45 uM PES              B    BC1   Silver salt + Add. Coat.sup.4                                    0       13 mm.sup.++    48 0.45 uM PES              B    BC2   Silver salt + Add. Coat.sup.4                                    0       13 mm.sup.++    49 0.45 uM PES              B    PSA   Silver salt + Add. Coat.sup.4                                    0       13 mm.sup.++    50 0.45 uM PES              B    BC1   Silver salt + Add. Coat.sup.5                                    0       13 mm.sup.++    51 0.45 uM PES              B    BC2   Silver salt + Add. Coat.sup.5                                    0       13 mm.sup.++    52 0.45 uM PES              B    PSA   Silver salt + Add. Coat.sup.5                                    0       13 mm.sup.++    53 0.45 uM PES              B    BC1   Silver salt + Add. Coat.sup.6                                    0       13 mm.sup.++    54 0.45 uM PES              B    BC2   Silver salt + Add. Coat.sup.6                                    0       13 mm.sup.++    55 0.45 uM PES              B    PSA   Silver salt + Add. Coat.sup.6                                    0       13 mm.sup.++    __________________________________________________________________________     A: *Johnson & Johnson HypoTears Tear Solution     B: B: Phosphate Buffered Saline     (P) Preservative containing     (PF) Preservative free     Uncoated: No coating, control     Silver coated: Coated with metallic silver     Silver + Additional coating:     .sup.1 Benzalkonium chloride thiol     .sup.2 poly(hexamethylenebiguanide) thiol     .sup.3 chainextended poly(hexamethylene biguanide) thiol     .sup.4 Silver halide + poly(hexamethylenebiguanide) overcoat     .sup.5 Silver iodide + poly(hexamethylenebiguanide) coating solution     .sup.6 Poly(hexamethylenebiguanide) coating followed by AgI/KI     introduction     Silver salt: Inorganic salt of silver halide salts such as chloride or     bromide or mixed salts of chloride, bromide and iodide     Avg. surface area of each bead: 0.07 cm.sup.2     Avg. surface area of each pellet: 0.02 cm.sup.2     BC1: Cocktail contains p. dimunuta, B. subtlis, and E. Coli     BC2: Cocktail contains S. aureus and p. aeruginosa     PD: Pseudomonmas dimunate     PSA: Pseudomonas aeruginosa     CA Candida albicans     **Stored for one month     .sup.+ Dimension of foil (cm)     .sup.++ Membrane diameter

Equivalents

Those skilled in the art will be able to ascertain, using no more thanroutine experimentation, many equivalents of the specific embodiments ofthe invention described herein. These and all other equivalents areintended to be encompassed by the following claims.

What is claimed is:
 1. A liquid composition for applying a non-leachableantimicrobial layer or coating on a surface, comprising a solution,dispersion or suspension of a biguanide polymer, a cross-linker reactedwith the biguanide polymer to form an adduct, and an antimicrobialmetal, metal salt or metal complex, wherein said metal, metal salt ormetal complex forms a complex with said adduct, and wherein saidantimicrobial layer or coating does not release biocidal levels ofleachables into a contacting solution.
 2. The liquid composition ofclaim 1 wherein the biguanide polymer is polyhexamethylene biguanide ora polymer containing biguanide moieties.
 3. The liquid composition ofclaim 1 wherein the metal is silver.
 4. The liquid composition of claim1 wherein the crosslinker is an organic compound containing reactivegroups selected from the group consisting of isocyanates, carboxylicacids, acid chlorides, acid anhydrides, succimidyl ether, aldehydes, andketones, or an organic compound selected from the group consisting ofalkyl methanesulfonates, alkyl trifluoromethanesulfonates, alkylparatoluenemethanesulfonates, alkyl halides and organic multifunctionalepoxides.
 5. The liquid composition of claim 1 wherein the metal salt issilver iodide.
 6. A liquid composition for applying an antimicrobiallayer or coating on a surface, comprising a solution, dispersion orsuspension of polyhexamethylene biguanide, a cross-linker reacted withthe biguanide polymer to form an adduct, and a silver salt, wherein saidsilver salt forms a complex with said adduct, and wherein saidantimicrobial layer or coating does not release biocidal levels ofleachables into a contacting solution.
 7. The liquid composition ofclaim 6 wherein the silver salt is silver iodide.
 8. The liquidcomposition of claim 6 further comprising wherein the polyhexamethylenebiguanide is reacted with an epoxide to form an adduct.
 9. The liquidcomposition of claim 8 wherein the epoxide is4,4'-methylene-bis(N,N-diglycidylaniline).